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Empty 28-03-2003 04:32 AM

Theoretical question for DIY yeast
 
"dGH" wrote in
:

If one were to connect an air pump and air stone INTO the 2L pop
bottle so that the yeast mixture was constantly bubbling: The net
effect would be that your output from the bottle would be CO2 enriched
air which continuously bubbled into the aquariium (or reactor). BUT,
the yeast reaction would never turn anaerobic, which means the product
would simply be CO2 and water. The yeast would never die, and the kit
would never have to be restarted or replaced. Theoretically you could
just add another cup of sugar each week to keep up the CO2 production.


The problem is not that the water and sugar turns anaerobic, but rather
that it turns alcoholic. As the proof goes up, the yeast starts dying off.
On top of that, adding air bubbles would no doubt cause surface turbulence,
which would hurt your CO2 levels as well.

~Empty
--
'You're not friends. You'll never be friends. You'll be in love till it
kills you both. You'll fight, and you'll shag, and you'll hate each other
till it makes you quiver, but you'll never be friends. Love isn't brains,
children, it's blood... blood screaming inside you to work its will. I may
be love's bitch, but at least I'm man enough to admit it.'
Spike

E. Mito 28-03-2003 06:56 PM

Theoretical question for DIY yeast
 
In article , Empty
writes:


The problem is not that the water and sugar turns anaerobic, but rather
that it turns alcoholic. As the proof goes up, the yeast starts dying off.
On top of that, adding air bubbles would no doubt cause surface turbulence,
which would hurt your CO2 levels as well.


I don't think adding air bubbles would decrease CO2 output; I've wanted to try
this very experiment to see how CO2 output would be affected but just never
gotten around to setting it up. Since yeast cultures grown up in a lab are
done so with constant agitation, I can only imagine that bubbling air into the
solution would only enhance growth. Alcohol production, however, is a problem,
and I'm guessing that if you enhance growth too much you might get increased
CO2 output but would deplete the yeast nutrients quicker. If you wanted to
prolong your culture you could just decant the nutrient-depleted supernatant
after CO2 generation drops and add a new sugar water solution -- the yeast
tends to sediment into an inactive pellet.

Erica
http://members.aol.com/_ht_a/mitoem/mitoem/index.htm


dGH 28-03-2003 07:20 PM

Theoretical question for DIY yeast
 

"E. Mito" wrote in
article , Empty
writes:


The problem is not that the water and sugar turns anaerobic, but rather
that it turns alcoholic. As the proof goes up, the yeast starts dying

off.
On top of that, adding air bubbles would no doubt cause surface

turbulence,
which would hurt your CO2 levels as well.


I don't think adding air bubbles would decrease CO2 output; I've wanted to

try
this very experiment to see how CO2 output would be affected but just

never
gotten around to setting it up. Since yeast cultures grown up in a lab

are
done so with constant agitation, I can only imagine that bubbling air into

the
solution would only enhance growth. Alcohol production, however, is a

problem,
and I'm guessing that if you enhance growth too much you might get

increased
CO2 output but would deplete the yeast nutrients quicker. If you wanted

to
prolong your culture you could just decant the nutrient-depleted

supernatant
after CO2 generation drops and add a new sugar water solution -- the yeast
tends to sediment into an inactive pellet.

Erica
http://members.aol.com/_ht_a/mitoem/mitoem/index.htm



Thanks for the feedback.
Perhaps I'm interpreting something incorectly, but from what I've read on
the net, alcohol isn't produced when the yeast are aerobic.



Tasslehoff 29-03-2003 10:56 AM

Theoretical question for DIY yeast
 



Thanks for the feedback.
Perhaps I'm interpreting something incorectly, but from what I've read on
the net, alcohol isn't produced when the yeast are aerobic.


I'm not certain about this but I think injecting air into the culture
increases the amount of yeast thus also increasing CO2 production and
shortening the available nutrients in a quicker amount of time, not to
mention wild Ph fluctuations.




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