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Old 25-10-2005, 06:21 PM
Andreas Giessauf
 
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Default DNA from fern species

Actually, I have purified a protein from plant material

of a true fern. From the pure protein a lot of its sequence

information has been obtained after tryptic digestion and

N-terminal sequence analysis of the resulting peptides

(about 70-80 % of the proteins amino acid sequence has been

determined).

By use of these amino acid sequences degenerative primers have

been deduced and pairs of primers from different peptides have

been used for PCR together with genomic DNA (after hundreds of

runs no meaningful PCR products have been obtained).

Until mow, the overexpression of only one recombinant fern

protein is described in literature and RNA was used (instead of

DNA) for construction of a cDNA library following PCR with

degenerative primers.

Does someone know is there a special intron or exon problem with

genomic DNA from fern species ?

Is the codon usage of this "fossil" plant family much different

to angiosperms or other organisms and the reason for the

problems ?

Any hints, help or comments to this problem is welcome.


Best regards,

Andreas Giessauf, PhD
Institut für Chemie
Universität Graz


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