Extracting chlorophyll, carotenoids, anthocyanin, etc
 

Greetings all
I am new to pigments and have this silly question. I want to estimate the amount
of different pigments of different pigments (Extracting chlorophyll, cartenoids,
antocyanin, betalains) in different parts of Pistacia sp. What I am looking for
is detailed though simple protocol (ideally no HPLC involved) that will give us
a rough estimate of the total amount of each group of pigments. Is there a way
to do differential extraction and then read the extract in spectrophotometer? I
know that how you do chlorophyll (in acetone?), but what about the rest? Is
there a better way?
Thanks
Yoram

Haifa Uninversity in Oranim
gerchman at research.haifa.ac.il



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Extracting chlorophyll, carotenoids, anthocyanin, etc
 

I just finished a lab in one of my university classes involving pigment
extraction. You can use acetone or 80% ethanol to extract the pigments.
Once chlorophyll is extracted everything else is too. Use
chromatography paper to separate out the pigments...the only separation
you might not get is the separation of Chl a and b. Once pigments are
separated on the chromo paper cut the strips and redissolve the
pigments. From there you can use your spec to get absorbance readings.

Hope that helps

Kyle