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Old 30-03-2006, 07:06 PM posted to bionet.plants
Roger Wise
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Default Postoctoral Positional - Functional Genomics of Plant Disease Defense

Postdoctoral Position Available
Iowa State University
Functional Genomics of Plant Disease Defense

A postdoctoral research position is available immediately in the Iowa
State University Center for Plant Responses to Enviromental Stresses
under the direction of Drs. Roger Wise and Steve Whitham. The
overall goals of the NSF-Plant Genome project "Functional Genomics of
Plant Disease Defense Pathways" are to
utilize functional genomics tools and resources available to barley,
rice, and the model dicot Arabidopsis thaliana, to accelerate
comparative analysis of disease defense pathways important in

Candidates will work with a team responsible for molecular, genetic,
and functional characterization of gene-specific sequences associated
with and required for R-gene mediated resistance responses using
barley powdery mildew as a model system. Approaches include
utilization of the 22K Affymetrix Barley1 GeneChip to characterize
the transcriptional networks regulated during powdery mildew induced
programmed cell death and subsequent high-throughput reverse genetic
systems in barley and Arabidopsis to functionally validate candidate
genes in resistance pathways of both monocot and dicot plants. All
of the above approaches will be facilitated by well-characterized,
high-resolution, recombinant mapping populations, accelerated
cell-death mutants, RNAi and TILLING silencing systems, BAC, cosmid,
and cDNA library resources.

Funding is available up to three years. Salary is commensurate with
experience. Ph.D. required in genetics, molecular biology, plant
physiology, plant pathology or related field. Experience in
bioinformatics/computational/statistical analysis, single-cell
transcript profiling, RNAi mediated gene silencing, cell biology,
and/or high-throughput gene mapping is desirable. Highly motivated
individuals should send curriculum vitae, research interests, and
reprints to the address below:

- Name, e-mail, address, phone, and fax no. of three references
should be included -

Dr. Roger Wise, USDA-ARS
Department of Plant Pathology
Iowa State University
Ames, IA, 50011-1020

Phone: 515-294-9756
Fax: 515-294-9420 ;

Recent Literatu
Shen, L, J Gong, RA Caldo, D Nettleton, D Cook, RP Wise, and JA
Dickerson. 2005. BarleyBase-an expression profiling database for
plant genomics. Nucleic Acids Research 33: D614-D618.

Caldo, RA, D Nettleton and RP Wise. 2004. Interaction-dependent gene
expression in Mla-specified response to barley powdery mildew. Plant
Cell. 16: 2514-2528.

Close, TJ, S Wanamaker, R Caldo, SM Turner, DA Ashlock, JA Dickerson,
RA Wing, GJ Muehlbauer, A Kleinhofs and RP Wise. 2004. A new resource
for cereal genomics: 22K barley GeneChip comes of age. Plant
Physiology 134: 960-968.

Halterman, D and RP Wise. 2004. A single amino acid substitution in
the sixth leucine-rich repeat of barley MLA6 and MLA13 alleviates
dependence on RAR1 for disease resistance signaling. Plant Journal
38: 215-226.

Iowa State University is an Equal Opportunity/Affirmative Action Employer.

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