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DEX-induced Chua Plasmid
Hello everyone,
I posted this same question to the methods mailing list a couple of days ago, and although I got some really interesting information, no one could really answer the question. So, here it goes again: Has anyone out there used Nam-Hai Chua's Dex-inducible plant expression vector (pTA7001) with Medicago truncatula? Chua himself does not seem to know whether or not it works with the species. I just want to be reassured before I go to the great trouble of obtaining and implementing this particular plasmid. One worry that a responder passed along is that there might be an endogenous chemical background in legumes that causes background induction, although this is unconfirmed for Medicago. Any thoughts? Thanks, Jim James J. Campanella, Associate Professor, Department of Biology and Molecular Biology Montclair State University 1 Normal Avenue Montclair, NJ 07043 Alternate email address: Ph: 973-655-4097 Fax: 973-655-7047 |
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