nos promoter in agro
Recently the construct was made were binary vector has spec resistance for
selection of E. coli containing this plasmid. Does anybody know if the nos promoter that is driving nptII (on T-DNA) is active in Agrobacterium? In our previous similar constructs nptII was driven by 35S not by nos promoter and Agro could be selected with kanamycin as well (100 mg/ml). I have read some references that nos promoter would be active but Agro could grow with kanamycin at lower concentration. Any comments or literature reference? Thanks |
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