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Old 06-05-2003, 07:44 PM
Cereoid-UR12
 
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Default quick and dirty total RNA quantitation from Cyanobacteria

Now, that's a really good gardening question.

Gardeners worry about that all the time while pulling weeds from their
flower beds!!!

Cyanobacteria aren't even plants!!!


wrote in message
.. .

I am looking for a quick and dirty way to get an estimate of total RNA

extracted from the cyanobacterium Phormidium autumnale. The RNA has been
extracted with a standard Phenol-Chloroform procedure and it is now frozen
in DEPC-trated water.

The purpose is just to get an estimate of total mRNA levels in P.

subjected to (drastically) different light treatments.

How can I quantify this RNA with minimal interference from DNA or free

bases, without having to go through complex image analysis or purification
procedures?

http://biowww.net/mynews/tree.php?gr...plants&begin=0




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