qPCR Newsletter - October 2008




Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

- single-cell handling - new page !
- single-cell PCR - pages updated !
- worldwide qPCR application workshops - register now !
- 2nd announcement = qPCR 2009 Event = http://www.qPCR2009.net
- online translation service of the Gene Quantification page to:
- CHINESE DUTCH FRENCH GERMAN GREEK
ITALIAN
- JAPANESE KOREAN PORTUGUESE RUSSIAN SPANISH

http://www.gene-quantification.asia
http://chinese.gene-quantification.asia/
http://dutch.gene-quantification.info/
http://french.gene-quantification.info/
http://german.gene-quantification.info/
http://greek.gene-quantification.info/
http://italian.gene-quantification.info/
http://japanese.gene-quantification.asia/
http://korean.gene-quantification.asia/
http://portuguese.gene-quantification.info/
http://russian.gene-quantification.asia/
http://spanish.gene-quantification.info/

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single-cell handling The purpose of this single-cell handling page is
to provide researchers and clinicians with workflows and resources for
single-cell molecular analysis such as RT-PCR, quantitative PCR, and
sequencing and with a focus on the front-to-backhandling of single
cells
= http://single-cell-handling.gene-quantification.info

Interest in single cell molecular analysis has risen dramatically over
the last couple of years, chiefly because single cell molecular
analysis is the only way to research genetic heterogeneity, i.e.,
differences in copy number or gene expression levels between
individual cells, or genetically analyze very rare cells such as
circulating tumor or fetal cells. Single cell molecular analysis has
provided new insight in diverse research areas such as immunology,
oncology, stem cells or forensics. However, an often overlooked aspect
of single cell analysis is the difficulties of handling single cells
for molecular analysis associate with all three major methods for
single cell manipulation:

- Micromanipulation
- LCM = Laser Capture Microdissection
- Flow cytometry
- Publications
- Interviews, Talks and Posters

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Single-cell handling papers

- New techniques for isolation of single prokaryotic cells. (REVIEW)
- Single cell sorting and cloning.
- Sampling efficiency of a single-cell capillary electrophoresis
system.
- Dynamic single cell culture array.
- Quantitative RT-PCR from one single cell using the AmpliGrid
technology.
- Low volume amplification and sequencing of mitochondrial DNA on a
chemically structured chip.
- Low-volume amplification on chemically structured chips using the
PowerPlex16 DNA amplification kit.
- http://single-cell-handling.gene-quantification.info

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UPDATE single-cell qPCR page The purpose of this single-cell PCR page
is to provide researchers with resources (papers, talks, posters) for
single-cell molecular analysis such as RT-PCR and quantitative PCR =
http://singlecell.gene-quantification.info/

New and innovative papers around single-cell qPCR:


Intracellular expression profiles measured by real-time PCR tomography
in the Xenopus laevis oocyte.
Quantification of mRNA in single cells and modelling of RT-qPCR
induced noise.
Transcription factor profiling in individual hematopoietic progenitors
by digital RT-PCR.
220-plex microRNA expression profile of a single cell.
MicroRNA quantitation from a single cell by PCR using SYBR Green
detection and LNA-based primers.
Split single-cell RT-PCR analysis of Purkinje cells.
The real-time polymerase chain reaction (a section with single-cell
qRT-PCR).
Single-cell gene expression profiling.
Gene expression and the myth of the average cell.
Combining laser capture microdissection with quantitative real-time
PCR: effects
of tissue manipulation on RNA quality and gene expression.
RNA amplification strategies for small sample populations.
Gene expression of single chondrocytes.
Gene expression profiling of individual bovine nuclear transfer
blastocysts.
Sensitive and quantitative measurement of gene expression directly
from a small amount of whole blood.
Expression profiling of small cellular samples in cancer: less is
more.


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With the new qPCR INFO PORTAL and all the presented tools we will help
you with to find the right information about qPCR and related topics
in Molecular Biology in the literature and in the World Wide Web.
= Papers / Protocols / Methods / Databases / Alets / Feeds / Books /
Forums / E-mail / Directory

http://infoportal.gene-quantification.info/


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Upcoming Events World-wide academic and commercial qPCR Events
http://events.gene-quantification.info/

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, ...etc..
Please submit your qPCR event here = events@gene-
quantification.info


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qPCR 2009 EVENT 9 - 13 March 2009

more news here = http://qPCR2009.net

download event FLYER = http://www.bioeps.com/qpcr2009/qPCR-...nouncement.pdf

It is a pleasure to announce the Nobel Prize Laureate Kary Mullis at
the qPCR 2009 event in an own session “25th Anniversary of PCR”

List of confirmed speakers = http://speakers.qpcr2009.net/
An industrial exhibition with the 30 world leading companies will be
held during the qPCR Symposium March 9-11 = http://exhibition.qpcr2009..net/
Our sponsors = http://sponsors.qpcr2009.net/

Please register = http://registration.qpcr2009.net/


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qPCR WORKSHOP

BioEPS GmbH / TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops, a
3-day qPCR Core Module and a 2-day qPCR Biostatistics Module. All
courses are held regularly in Göteborg, Sweden, in English and in
Freising-Weihenstephan, Germany, in German and English, and in Prague,
Czech Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and specialized
trainings will be held as well across Europe and world-wide.
TATAA Biocenter Germany workshops are held in cooperation with BioEPS
GmbH, located at the campus of the Technical University of Munich, in
Freising-Weihenstephan, very close to the Munich Airport (MUC). For
more information and registration, please see our web page
= http://TATAA.gene-quantification.info/


Course Occasions 2008/2009:
3-day qPCR Core Module (Mon. - Wed.)
2-day BioStatistics Module (Thu. - Fri.)
single-cell qPCR Module (Mon. - Wed.)

27-31 Oct Göteborg qPCR Core Module + HRM + Biostatistics
17-21 Nov Prague qPCR Core Module + Practical Biostatistics
24-28 Nov Freising Germany qPCR Core Module + Biostatistics
(English language)
1-5 Dec Göteborg qPCR Core Module + Biostatistics
8 - 10 December 2008 (E) NEW singel-cell qPCR Application Workshop
15-19 Dec Prague RNA Isolation + Expression Profiling and Data
Analysis
26 - 30th January 2009 in Freising, Germany, English language


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Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !


Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
http://www.gene-quantification.info


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