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vekcna

28-07-2003 02:36 PM

arabidopsis nuclei isolation

I’m looking for a good method for arabidopsis nuclei isolation
So far I tried using buffer containing hexylene glycol and 0.2% triton For percoll gradient and couldn’t see shi%$#... in the 80 % layer.
I suspect that somehow I damaged the nuclei
If anyone has any idea of what is wrong or an alternative method I would be grateful.

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