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Role of RNA Silencing in Plants
I want to know exatly How the process of RNA silencing is used by the
plants against the invading viruses? What all proteins and genes regulate the activity of RNA silencing. Does mutations in the gene sequences result in inhibition of the activity and make the plant more suceptible to various kinds of plant viruses? How does the knowledge of RNA Silencing in plants help us in develpoing new viral resistant varities ? |
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Role of RNA Silencing in Plants
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#3
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Role of RNA Silencing in Plants
If this is a school or uni question you should have access to bids/web
of science/ science direct and be able to find the current articles if not try scirus. if memory serves the current hypothesis is that plants are able to take advantage of the fact that a lot of plant viral pathogens have a double stranded RNA genome. The double stranded RNA is cut up in to ~23bp bits by a DICER (?) enzyme, these bits are used to prime binding of another RNAse so and single stranded RNA is also rendered untranslatable. This silencing ability is able to spread from the site of initial activation thus protecting the new plant growth (i don't remember if it spreads to existing tissue). look up: PTGS - post transcription gene silencing, siRNA short interfering RNAs i've just found a useful article - Waterhouse et al TRENDS in plant science vol 6 no 7 2001 p297 How does the knowledge of RNA Silencing in plants help us in develpoing new viral resistant varities ? depends on the plant and it's uses. in theory you could have a varetiy of conserved viral sequences constitutively expressed as hair pins (will make a double stranded RNA on their own) so the virus can't get a 'foot hold' and do damage with infection. however this would cost the plant. If you happen to be a faceless multinational who wants to generate some transgenic monoclonal crop plant then some version of this would probably be what your looking for as it would be easy to make (depend on transformation efficiency of the plant of course). there would be more elegant methods - inducible systems linked to a transcriptional activator (gal4 ?) and kicking out a variety of hairpins then shutting down when not in use. although being a thing of beauty such system would probably never get funding because it would take at least twice as long to make then you should do lengthy trails as any transgene could affect the metabolic profile of the plant in an unpredictable way. grrr. ops. time for me to have a little sit down. craig |
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