protein extraction from protplasts ?
I need some information regarding extraction of protein from
transfected tomato protoplasts. I am using 2x10(4) cells for PEG
I tried sonication, micropestle grinding, freeze-thawing,... but none
of these seem to work. I tried to estimate protein concentration by
BCA protein assay and Biorad protein assay. I got very low or negative
Does anybody know
1. how exactly protein is extracted from protoplasts
2. how many protoplasts are needed for extraction
I need to do immunoprecipitation and RT-PCR experiments.
Could somebody give me some insights into this.
Thanks a lot in advance
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