"Al" wrote in message ...
I am looking into a piece of lab equipment called a roller drum. How fast
does the drum need to turn for best results?
I have identified two models. The first model, TC-7, has a rotation speed
of 6.0 - 56.0 rpm and the second model, TC-8, has a rotation speed of 0.2 -
3.5 rpm.
http://www.nbsc.com/products/rollerdrums/Default.asp
It seems to me that the spin would not need to be faster than a constant 2
or 3 rotations per minute which sounds about right if the goal is to keep
the tissue in the liquid media from orienting itself to gravity and light
direction. A rotation of 6 times a minute sounds fast to me and a top speed
of 56 rpm must be wrong for this purpose, but I don't know. Does anyone
reading this have any experience that could help me choose correctly?
Thanks,
Al
Hi Al, I personally use a New Brunswick shaker table. I have a wheel
but it's not N.B. The shaker is super (I'm not necessesarily
recomending shaker) really trying to say New Brunswick stuff is the
best on the market IMHO. The 2 or 3 rpm is fine --you only need to
keep the tissue disoriented so that it will multiply rather than put
down roots & put up a shoot. I suppose you know you need constant
light while 'cloning ?? (just threw that in) G Good Luck, Bill