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Old 15-04-2005, 04:32 PM
some body
 
Posts: n/a
Default DNA isolation from old leaves

Hi everybody,
I am having trouble isolating DNA from oak leaves that
went from the tree into the -80C freezer about one
year ago. Nothing else has been done with them. I am
using the Qiagen Plant DNA kit so far, but PCR works
only occasionally. Does somebody have a DNA isolation
protocol which might give a better chance of getting
PCR products?
thanks a lot for your help,

Manuel



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