Hi Everyone,
You might want to check out the Cartagen DNA Extraction Kit for Plants. It combines a CTAB method with a column clean up step. They also have some kits for going straight to PCR that have been used on oak leaves. Their product info is at
www.cartgen.com.
dwm
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Originally Posted by some body
Hi everybody,
I am having trouble isolating DNA from oak leaves that
went from the tree into the -80C freezer about one
year ago. Nothing else has been done with them. I am
using the Qiagen Plant DNA kit so far, but PCR works
only occasionally. Does somebody have a DNA isolation
protocol which might give a better chance of getting
PCR products?
thanks a lot for your help,
Manuel
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