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Old 24-06-2005, 07:02 PM
Aaron Hicks
 
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And once you DO have the seeds, knock that MS back to 1/2
strength, put sugar at 20 grams per liter, and have at it. They'll
germinate quite readily on that.

Orchids are "replated" (versus "subcultured" for normal plants),
and the medium that is employed is similar to 1/2 MS + 2% sucrose, but
with 20-30 grams of "other" stuff- usually an undefined component such as
coconut liquid, pineapple juice, or overripe bananas. You can knock back
MS to as little as 35% label strength with sucrose at 1.4%, and then
coconut or pineapple at 50-100 mL/L, and then agar at 8-10 grams per
liter, depending upon the type used.

Or you can just buy something like O156 from PhytoTech, and use
that at 70-80% label strength, with about 8.5 to 9 grams of their A111
agar per liter.

The email address in the header doesn't work. Send no mail there.

Cheers,

-AJHicks
Chandler, AZ