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Enzymatic hydrolysis of cellulose- How do you do it with off the shelf stuff ?
Enzymatic hydrolysis of cellulose- How do you do it with off the
shelf stuff ? Appologies if this is the wrong forumn but hopefully someone can steer me in the right direction. I have been looking into ways of converting cellulose into Ethanol to run the car as fuel prices are becoming annoying. The problem is I don't know a anything about biochemistry. Having said this I have read as much as I can and would like to give this "Enzymatic hydrolysis of cellulose" a go. From what I understand the following is the case : Cellulose is a long chain of laminar pollysaccarides. Specifically Glucose. Because of the tightly packed nature and composition of cellulose you need several enzymes to b reak it down ; 1. An endoglucanase - random chomps at the chain producing new ends 2. An exoglucanase - a progressive chomp 3. A ß-glucosidase - a break down of the broken bits further to D-Glucose Now not being a biochemist I am kind of hoping someone out there knows what all this means and has a shopping list of stuff that will equate. Anyone out there know where to get all this stuff or how to do this at the laymans level ? Any advice greatly appreciated ... Steve |
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