The Reference in qPCR - Academic & Industrial Information Platform


Dear researcher,
dear Gene Quantification page reader,


The Gene Quantification page - www.Gene-Quantification.info -
describes and summarises all technical aspects involved in
quantitative gene expression analysis using real-time qPCR & qRT-PCR.
It presents a lot of applications, chemistries, methods, algorithms,
cyclers, kits, dyes, analysis methods, meetings, workshops, and
services involved. Commercial and academic institutions can present
their qPCR tools right here

= http://qpcrplatform.gene-quantification.info/


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The quantification strategy in real-time RT-PCR is the principal
marker in gene quantification. Generally two strategies can be
performed in real-time RT-PCR. The levels of expressed genes may be
measured by absolute quantification or relative quantitative real-time
RT-PCR.
Absolute quantification relates the PCR signal to input copy number
using a calibration curve, while relative quantification measures the
relative change in mRNA expression levels. The reliability of an
absolute real-time RT-PCR assay depends on the condition of
'identical' amplification efficiencies for both the native target and
the calibration curve in RT reaction and in following kinetic PCR.
Relative quantification is easier to perform than absolute
quantification because a calibration curve is not necessary. It is
based on the expression levels of a target gene versus a housekeeping
gene (reference or control gene) and in theory is adequate for most
purposes to investigate physiological changes in gene expression
levels. The units used to express relative quantities are irrelevant,
and the relative quantities can be compared across multiple real-time
RT-PCR experiments.

= http://strategy.gene-quantification.info/


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With this page and all the presented tools we will help you with to
find the right information about qPCR and related topics in Molecular
Biology in the literature and in the World Wide Web:
New Papers / Protocols / Methods / Databases / Alets / Feeds / Books /
Forums / E-mail / Directory

= http://infoportal.gene-quantification.info/


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Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of the December newsletter issue
was:

= Copy Number Variation
= new webinars online
= new application workshops in 2008
............

http://qpcrnews.gene-quantification.info/


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The gene copy number (also "copy number variants" or CNVs) is the
number of copies of a particular gene in the genotype of an
individual. Recent evidence shows that the gene copy number can be
elevated in cancer cells.

= Why are CNVs important?

Differences in the DNA sequence of our genomes contribute to our
uniqueness. These changes influence most traits including
susceptibility to disease. It was thought that single nucleotide
changes (called SNPs) in DNA were the most prevalent and important
form of genetic variation. The current studies reveal that CNVs
comprise at least three times the total nucleotide content of SNPs.
Since CNVs often encompass genes, they may have important roles both
in human disease and drug response. Understanding the mechanisms of
CNV formation may also help us better understand human genome
evolution.

http://cnv.gene-quantification.info/


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Data Analysis and BioInformatics in real-time qPCR

Bioinformatics is a multidisciplinary approach to discribe, model and
understand biological processes on basis of information on genes,
proteins and metabolism. It uses computers, data bases and algorythms
to link information and translate it back into biology, physiology or
pathophysiology.

BioInformatics = Database Management Systems, Data Mining, Sample
Tracking, Information Management, Data Acquisition, Data Analysis,
Statistics, Pattern Recognition & Classification, Simulation &
Modeling

Bioinformatics initially centered on sequence and genome analysis but
now the extensive use of microarrays, mass spectrometry, qPCR and qRT-
PCR, has stimulated bioinformatic work in data acquisition, signal
processing, and data mining. Also, simulation and modeling are
becoming increasingly important areas of focus in bioinformatics which
finally will lead to a new level of understanding the networks in the
metabolism: Genomics, Transcriptomics, Splicomics, Proteomics,
Metabolomics, etc.

http://bioinformatics.gene-quantification.info/


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TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops, a
3-day qPCR Core Module and a 2-day qPCR Biostatistics Module. All
courses are held regularly in Göteborg, Sweden, in English and in
Freising-Weihenstephan, Germany, in German and English, and in Prague,
Czech Republic in English and Czech.
http://tataa.gene-quantification.info/

Course Occasions 2007 and 2008:
3-day qPCR Core Module (Mon. - Wed.) and 2-day BioStatistics
Module (Thu. - Fri.)

= 3rd - 7th March 2008 (in Freising, Germany, English language)
= 5 - 9th May 2008 (in Freising, Germany, Kurs wird in DEUTSCH
gehalten, German language)
= 7 - 11th July 2008 (in Freising, Germany, English language)

= Please register here = http://www.tataa.com/Courses/Courses.html


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Forward Please send the mail to further scientists and friends who are
interested in qPCR !


Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
http://www.Gene-Quantification.info



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