protoplast transfection
 

Hello colleagues

I'm a PhD student in plant molecular biology.
I'm trying to do an assay where i need to electroporate tomato
protoplasts and also test for reporter gene activity. I am really
running out of time and i need some immediate help

I'm able to get the protoplasts from both leaves and suspension cells
, but i am not able to keep them alive after electroporation. I'm
using ECM 830 (BTX) electroporator.

I need some information regarding the electroporation conditions for
tomato protoplasts using this machine and also GUS reporter gene assay
and anyother information that would keep my protoplasts alive and
uptake the DNA.

Any help in this regard will be very much appreciated.

Thank you in advance