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protoplast transfection
Hello colleagues
I'm a PhD student in plant molecular biology. I'm trying to do an assay where i need to electroporate tomato protoplasts and also test for reporter gene activity. I am really running out of time and i need some immediate help I'm able to get the protoplasts from both leaves and suspension cells , but i am not able to keep them alive after electroporation. I'm using ECM 830 (BTX) electroporator. I need some information regarding the electroporation conditions for tomato protoplasts using this machine and also GUS reporter gene assay and anyother information that would keep my protoplasts alive and uptake the DNA. Any help in this regard will be very much appreciated. Thank you in advance |
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