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Controlling in vitro bacterial growth without affecting fungus?
Hey everyone. Thanks to everyone who offered advice on how to make thin
sections of thalli. I ended up using my forceps as a crude vise to hold the thallus in position, and I used a sharp, thin, steel razor blade to slice it. The added advantage of the forceps was that they acted as a guide. All of this was done under a dissecting microscope. Anyway, I made some really nice slides, and although they weren't quite as thin as I would hope, I had nice clear cross-sections of lichen thalli. I even observed ascospores, and hopefully I'll be able to use the ascospore morphology to help identify the lichen. If I had a camera I'd post pictures for you! Anyway, I'm currently working on an experimental design and I have this idea to grow an isolated, but contaminated mycobiont from a lichen - that is, isolated from the algal photobiont and contaminated with any bacteria that may be already living within the thallus. The problem here is that I'm afraid the bacterial population will get out of control. I'm brainstorming control measures, and my best idea so far is to use an antibiotic to hinder bacterial population growth. I'm also considering altering ambient conditions, like temperature, but I do not want to detrimentally affect the fungus in any way. Essentially, I want the fungus to grow in the presence of bacteria for a number of weeks, without ending up with huge bacterial colonies dot the medium and a little smudge of fungus. If anyone has ideas I will very much appreciate it! -Nitrobacter |
#2
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Controlling in vitro bacterial growth without affecting fungus?
"Nitrobacter" wrote in message
rs.com... Hey everyone. Thanks to everyone who offered advice on how to make thin sections of thalli. I ended up using my forceps as a crude vise to hold the thallus in position, and I used a sharp, thin, steel razor blade to slice it. The added advantage of the forceps was that they acted as a guide. All of this was done under a dissecting microscope. Anyway, I made some really nice slides, and although they weren't quite as thin as I would hope, I had nice clear cross-sections of lichen thalli. I even observed ascospores, and hopefully I'll be able to use the ascospore morphology to help identify the lichen. If I had a camera I'd post pictures for you! Anyway, I'm currently working on an experimental design and I have this idea to grow an isolated, but contaminated mycobiont from a lichen - that is, isolated from the algal photobiont and contaminated with any bacteria that may be already living within the thallus. The problem here is that I'm afraid the bacterial population will get out of control. I'm brainstorming control measures, and my best idea so far is to use an antibiotic to hinder bacterial population growth. I'm also considering altering ambient conditions, like temperature, but I do not want to detrimentally affect the fungus in any way. Essentially, I want the fungus to grow in the presence of bacteria for a number of weeks, without ending up with huge bacterial colonies dot the medium and a little smudge of fungus. If anyone has ideas I will very much appreciate it! -Nitrobacter Increased osmotic strength and reduced pH (individually or together) normally favour fungal growth while not completely preventing bacterial growth. GS |
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