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Paying to find non-GE wild corn?
David Kendra wrote:
"Brian Sandle" wrote in message ... David Kendra wrote: "Brian Sandle" wrote in message ... Epithelial cells. It looks like the whole article is free to read: Linkname: J. Bact -- Ferguson et al. 184 (8): 2235 URL: http://jb.asm.org/cgi/content/full/1...&pmid=11914355 size: 947 lines The cells are artificially cultured in vitro. How do they compare to true non-dividing epithelial cells? Thanks. I thought the epithelial cells, such as line the gut, would be frequently dividing. The epithelial cells in our guts do in deed divide albeit at a slower rate than in vitro culture. Perhaps more importantly they are sluffed off after a period of time as part of the natural process. So have to divide quickly to be replacing. Cells in culture can survive for extended periods of time as long as fresh media is added and toxic metabolites removed. So first you complain there is a difference because you say they are not living long, then you complain because you say they are living long. Much bigger differences in GM food. Search for Schubbert R for some info on the fate of the foreign DNA. I am well aware of work on foreign DNA. It is a natural process that takes place all the time So do you or do you not believe foreign DNA is brken down in the gut? - so in essence, ALL DNA is GE!!!! DNA varies, but GE DNA cheats. What is your take on this work? U.S. National Library of Medicine Gateway On the fate of orally ingested foreign DNA in mice: chromosomal association and placental transmission to the fetus. Schubbert R, Hohlweg U, Renz D, Doerfler W. Mol Gen Genet. 1998 Oct;259(6):569-76. Institute of Genetics, University of Cologne, Koeln, Germany. We have previously shown that, when administered orally to mice, bacteriophage M13 DNA, as a paradigm foreign DNA without homology to the mouse genome, can persist in fragmented form in the gastrointestinal tract, penetrate the intestinal wall, and reach the nuclei of leukocytes, spleen and liver cells. Similar results were obtained when a plasmid containing the gene for the green fluorescent protein (pEGFP-C1) was fed to mice. In spleen, the foreign DNA was detected in covalent linkage to DNA with a high degree of homology to mouse genes, perhaps pseudogenes, or to authentic E. coli DNA. We have now extended these studies to the offspring of mice that were fed regularly during pregnancy with a daily dose of 50 microg of M13 or pEGFP-C1 DNA. Using the polymerase chain reaction (PCR) or the fluorescent in situ hybridization (FISH) method, foreign DNA, orally ingested by pregnant mice, can be discovered in various organs of fetuses and of newborn animals. The M13 DNA fragments have a length of about 830 bp. In various organs of the mouse fetus, clusters of cells contain foreign DNA as revealed by FISH. The foreign DNA is invariably located in the nuclei. We have never found all cells of the fetus to be transgenic for the foreign DNA. This distribution pattern argues for a transplacental pathway rather than for germline transmission which might be expected only after long-time feeding regimens. In rare cells of three different fetuses, whose mothers have been fed with M 13 DNA during gestation, the foreign DNA was detected by FISH in association with both chromatids. Is maternally ingested foreign DNA a potential mutagen for the developing fetus? And I bet if there is any more info on this it will be unlikely to get published easily. Who goes out bragging about their troubles? |
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