Thread: Algae Question
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Old 27-01-2006, 09:47 PM posted to sci.bio.botany
rene
 
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Default Algae Question

In a fluorescence microscope with near UV excitation (pretty standard
in labs) whatever thing with chloroplasts fluoresces deep red. For DIY
you will need at least a very strong lamp (a slide projector will do)
and suitable filters: the exciter, more or less near UV, and a blocking
filter that makes sure you block the UV and all remnant deep blue. And
at the same time let's through as much as possible of the longer
wavelength. UV-leds are another promising possibility, but I haven't
experimented with it.
Make sure you give it at least 10 min in a fully darkened room before
you give up: the efficiency is low and you need time to adapt to the
low light.

Rene.