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Algae Question
I've been reading a book about algae, several places it mentions
fluorescence from the accessory pigments. what would be required to see this fluorescence? I have a microscope, and believe I could get somewhat narrow band light using a light source and a prism. |
#2
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Algae Question
Hi,
"Charles" wrote in message ... I've been reading a book about algae, several places it mentions fluorescence from the accessory pigments. what would be required to see this fluorescence? I have a microscope, and believe I could get somewhat narrow band light using a light source and a prism. Fluorescence generally means that the item absorbs a high energy photon and emits a lower energy photon rapidly. Usually in common applications this means you shine a black light (UV-ultraviolet) on it and it emits a visible photon. The black lights are generally mercury vapor lamps which emit several very sharp lines, no need for a prism, a long wave at I think around 350 nm, and a short wave around 254nm (quite damaging to the eye). Google on black light and you will find lots of suppliers for many hobby applications. The two lines excite different fluorescence. The short wave is generally not transmitted through normal glass, the light bulb is quartz. You would probably illuminate the sample without any intermediate plain glass lens and then could look at the fluorescing visible photons normally through the microscope. You would arrange the lighting so you are not staring at the UV light with either eye,and the microscope optics would not let the most dangerous UV get to your eye. Hope that helps, Rick |
#3
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Algae Question
Hi Charles,
Many of the pigments in plants and algae fluoresce. In particular - chlorophyll. As a test for water quality, chloropyll fluorescence is used to detect certain parameters. (Chlorophyll fluorescence detected, must mean there is algae in the water). To see the fluorescence you need to excite the pigment with the correct frequency of light. In most cases, the amount of fluorescence is not easily detected by the unaided eye. Instruments made to detect the fluorescence utilize photo diodes, avalanche diodes and photo multiplyer devices (for extreme low level detection). You can however use a filter set to allow the fluoresced light frequence pass through while blocking other frequencies thus enabling you to "see" it. You are probably most familiar with UV or black light being the excitation source at non-visable wavelengths and then seeing the fluoresced result at visible wavelengths. Chlorophyll is excited by light in the visible spectrum as well as many other pigments. You can look up the excitation frequencies of these compounds and see for yourself. Many may have a number of excitation wavelengths, some stronger than others. I hope this is of help. Larry T. Charles wrote: I've been reading a book about algae, several places it mentions fluorescence from the accessory pigments. what would be required to see this fluorescence? I have a microscope, and believe I could get somewhat narrow band light using a light source and a prism. |
#4
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Algae Question
UV fluorescence I am familiar with, what I was thinking at the time
was picking up some from pigments that absorb in the visual spectrum. Things like carotenoids and phycobilins. It may be that the fluorescence is too weak to see with home apparatus and only absorption would be measurable. Just wondering. Thanks |
#5
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Algae Question
In a fluorescence microscope with near UV excitation (pretty standard
in labs) whatever thing with chloroplasts fluoresces deep red. For DIY you will need at least a very strong lamp (a slide projector will do) and suitable filters: the exciter, more or less near UV, and a blocking filter that makes sure you block the UV and all remnant deep blue. And at the same time let's through as much as possible of the longer wavelength. UV-leds are another promising possibility, but I haven't experimented with it. Make sure you give it at least 10 min in a fully darkened room before you give up: the efficiency is low and you need time to adapt to the low light. Rene. |
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