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RNA
Hi,
I'm affraid I can't see the picture you refer below. But usually the bands above the wells refer to some kind of protein contamination. This could interfer with the reverse transciptase process to obtain your cDNA. What you could do, is to extract your RNA band from the gel before constructing your cDNA. This way your RNA would be free from the contamination. Best whishes, Pfrogs wrote in message ... I am extracting RNA from rice leaves using Trizol . The RNA looks like good but I got a bright band that run above the wells. I do not know what is that. I hope you can see the picture below. I will construct a cDNA library with this RNA but I am afraid this bright band can mess up everything. Could you please give me your opinion in this respect? Thank you for your attention. Angela http://biowww.net/mynews/tree.php?gr...plants&begin=0 |
#2
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|||
RNA
Hi,
I'm affraid I can't see the picture you refer below. But usually the bands above the wells refer to some kind of protein contamination. This could interfer with the reverse transciptase process to obtain your cDNA. What you could do, is to extract your RNA band from the gel before constructing your cDNA. This way your RNA would be free from the contamination. Best whishes, Pfrogs wrote in message ... I am extracting RNA from rice leaves using Trizol . The RNA looks like good but I got a bright band that run above the wells. I do not know what is that. I hope you can see the picture below. I will construct a cDNA library with this RNA but I am afraid this bright band can mess up everything. Could you please give me your opinion in this respect? Thank you for your attention. Angela http://biowww.net/mynews/tree.php?gr...plants&begin=0 |
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