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#1
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Autoclave
Anybody here have instructions on how to turn a pressure cooker into an
autoclave??? Kye. - Who is going to start flasking after he builds his Laminar Flow Cabinet |
#2
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Autoclave
Good news Kye, your pressure cooker already is an autoclave. :-)
Run it at full pressure for at least 20 minutes. You might want to run a flask or 2 or 3 but don't open them or use any seeds. If nothing bad grows, you should be all set. If something grows on the medium, you either need a longer time or higher Temperature/pressure. Steve PS Welcome back to the newsgroups, again. Kye wrote: Anybody here have instructions on how to turn a pressure cooker into an autoclave??? Kye. - Who is going to start flasking after he builds his Laminar Flow Cabinet |
#3
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Autoclave
Sweet... Thanks for the details...
Anybody got an affordable media recipe I can try??? Kye. PS... With or without lids??? "Steve" wrote in message . .. Good news Kye, your pressure cooker already is an autoclave. :-) Run it at full pressure for at least 20 minutes. You might want to run a flask or 2 or 3 but don't open them or use any seeds. If nothing bad grows, you should be all set. If something grows on the medium, you either need a longer time or higher Temperature/pressure. Steve PS Welcome back to the newsgroups, again. Kye wrote: Anybody here have instructions on how to turn a pressure cooker into an autoclave??? Kye. - Who is going to start flasking after he builds his Laminar Flow Cabinet |
#4
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Autoclave
Kye wrote: .................... With or without lids??? With lids. Exactly as you will take them to your new Laminar Flow Cabinet. When the time comes to grow seeds, you will open them there and "install" the seeds, and reclose, right? To test the pressure cooker, don't open them at all. If no fungus or anything grows, the pressure cooker passed its test. Later you can test your technique in the Laminar Flow Cabinet to see if you pass also. By the way, I am replying based on some knowledge of autoclaves and pressure cookers. Perhaps someone who has actually used a pressure cooker in flasking orchid seeds can confirm what I say or add to it? Steve |
#6
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Autoclave
Hi
By the way, I am replying based on some knowledge of autoclaves and pressure cookers. Perhaps someone who has actually used a pressure cooker in flasking orchid seeds can confirm what I say or add to it? I'm using a pressure cooker for more than one year now, 20 min at full pressure, never got any fungus (and I autoclaved more than 400 bottles). But I never sowed directly after autoclaving, I always waited one week to see, if anything is starting to grow. Philipp |
#7
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Autoclave
Steve wrote:
Kye wrote: .................... With or without lids??? With lids. Exactly as you will take them to your new Laminar Flow Cabinet. When the time comes to grow seeds, you will open them there and "install" the seeds, and reclose, right? To test the pressure cooker, don't open them at all. If no fungus or anything grows, the pressure cooker passed its test. Later you can test your technique in the Laminar Flow Cabinet to see if you pass also. By the way, I am replying based on some knowledge of autoclaves and pressure cookers. Perhaps someone who has actually used a pressure cooker in flasking orchid seeds can confirm what I say or add to it? Steve What he said... But don't tighten on the lids. Things explode if you do that (or implode after cooling). I've only ever used a pressure cooker for pressure canning, but the principle is the same for autoclaves and pressure cookers. Put the lids on loosely, and tighten them down after the media cools. If you want to add heat labile supplements (some vitamins, for example), you need to prepare them sterilely and add them before the agar sets. I used to let autoclaved media cool in my flasking hood, with the lids partially off, actually. Limits implosions and keeps condensation down (so that you can at least see into your flasks). But you can cool them (lidded) on the counter if you want, or if you have time let them cool down in the pressure cooker. That will take a long time... You don't need a laminar flow hood, but it certainly helps. And I agree completely about testing your technique. Far better to practice with a few flasks than make a hundred and find out you screwed up the first step. _YOU WILL GET CONTAMINATION_ regardless. Anybody that says they are 100% contamination free is not telling you the entire truth or hasn't done enough flasking. Eventually, something will slip through. The goal is to start with good technique, clean equipment, and sterile media, so as to limit your contamination problems as much as possible. Speaking of media recipies, there is an old one that is just fertilizer, agar, and sugar (I think). Does anybody have a recipe for that? Rob -- Rob's Rules: http://www.msu.edu/~halgren 1) There is always room for one more orchid 2) There is always room for two more orchids 2a. See rule 1 3) When one has insufficient credit to purchase more orchids, obtain more credit |
#8
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Autoclave
DOES SOMEBODY HAS USED THIS MODEL, I'M THINKING ABOUT BUYING IT
http://www.pressurecooker-outlet.com/921.htm Larry Dighera wrote: On Fri, 19 Dec 2003 00:01:47 GMT, "Kye" wrote in Message-Id: : Anybody here have instructions on how to turn a pressure cooker into an autoclave??? 20 minutes at 15 PSI, IIRC. Kye. - Who is going to start flasking after he builds his Laminar Flow Cabinet Here are a few links that you may find useful in your construction project: http://www.geocities.com/~marylois/arch207.html http://www.fungifun.com/flowhood/ http://www.fungifun.com/flowhood/match.htm http://www.filtersrx.com/sizes.asp?CAT=HEPHP9 http://www.hepafilters.com/CFS/filte...rance_sale.htm http://www.llnl.gov/es_and_h/hsm/doc.../doc12-05.html http://www.grainger.com/Grainger/pro...mId=1611630999 http://www.surpluscenter.com/sort.as...03061222210572 http://www.surpluscenter.com/item.as...tname=electric http://www.micromagazine.com/company...kstations.html http://cleanroomservice.com/products.htm http://terrauniversal.com/products/h...cleanbth.shtml http://www.rayplastics.com/micronairetext.html http://www.jaroca.com/ http://www.micromagazine.com/company/co/115/11591.html http://techrite.com/ceilingfilterhome.html http://techrite.com/lamproducthome.html http://www.geniescientific.com/genieflo.html |
#9
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Autoclave
On Fri, 19 Dec 2003 23:46:51 GMT, AN wrote in
Message-Id: : DOES SOMEBODY HAS USED THIS MODEL, I'M THINKING ABOUT BUYING IT http://www.pressurecooker-outlet.com/921.htm Consider eBay and save: http://search.ebay.com/search/search...axPrice=&ebayt ag1code=0&st=&SortProperty=MetaEndSort |
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