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#1
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Plant tissue cloning?
Any good books or web sites with info on cloning plants from smallish tissue samples at home? I'm
interested in trying to clone some rare-ish/expensive-ish venus flytrap and pitcher plant cultivars. Back in High School, I tried and failed cloning African Violets using one of the educational cloning kits that were just coming onto the market at the time. I've found references to several "cloning gel" products (mostly, apparently, on pothead sites). How useful would a product like this: http://www.hydroponics-garden.com/rootechgel4oz.html be for my purposes? My main worry is getting bacterial/fungal contamination in the medium-- and this claims to have "antimicrobials" in the medium. |
#2
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Plant tissue cloning?
I would try the American patent office at first - there is a section on
plants and cultivars - at http://patft.uspto.gov/netahtml/search-adv.htm These patents also have references to other patents and texts. "Darren Garrison" wrote in message ... Any good books or web sites with info on cloning plants from smallish tissue samples at home? I'm interested in trying to clone some rare-ish/expensive-ish venus flytrap and pitcher plant cultivars. Back in High School, I tried and failed cloning African Violets using one of the educational cloning kits that were just coming onto the market at the time. I've found references to several "cloning gel" products (mostly, apparently, on pothead sites). How useful would a product like this: http://www.hydroponics-garden.com/rootechgel4oz.html be for my purposes? My main worry is getting bacterial/fungal contamination in the medium-- and this claims to have "antimicrobials" in the medium. |
#3
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Plant tissue cloning?
I would try the American patent office at first - there is a section on
plants and cultivars - at http://patft.uspto.gov/netahtml/search-adv.htm These patents also have references to other patents and texts. "Darren Garrison" wrote in message ... Any good books or web sites with info on cloning plants from smallish tissue samples at home? I'm interested in trying to clone some rare-ish/expensive-ish venus flytrap and pitcher plant cultivars. Back in High School, I tried and failed cloning African Violets using one of the educational cloning kits that were just coming onto the market at the time. I've found references to several "cloning gel" products (mostly, apparently, on pothead sites). How useful would a product like this: http://www.hydroponics-garden.com/rootechgel4oz.html be for my purposes? My main worry is getting bacterial/fungal contamination in the medium-- and this claims to have "antimicrobials" in the medium. |
#4
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Plant tissue cloning?
On Wed, 3 Mar 2004 11:09:36 +1100, "Peter Jason" wrote:
I would try the American patent office at first - there is a section on plants and cultivars - at http://patft.uspto.gov/netahtml/search-adv.htm These patents also have references to other patents and texts. Frankly, I don't give even a TINY fraction of a damn if a plant has a patent on it. If I have one, want more, and can make more (for my own personal use), I'll make more. Period. |
#5
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Plant tissue cloning?
On Wed, 3 Mar 2004 11:09:36 +1100, "Peter Jason" wrote:
I would try the American patent office at first - there is a section on plants and cultivars - at http://patft.uspto.gov/netahtml/search-adv.htm These patents also have references to other patents and texts. Plus, I find the very IDEA of patening a plant or animal to be utterly idiotic-- and I don't care to hear about "business models". |
#6
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Plant tissue cloning?
On Wed, 3 Mar 2004 11:09:36 +1100, "Peter Jason" wrote:
I would try the American patent office at first - there is a section on plants and cultivars - at http://patft.uspto.gov/netahtml/search-adv.htm These patents also have references to other patents and texts. Frankly, I don't give even a TINY fraction of a damn if a plant has a patent on it. If I have one, want more, and can make more (for my own personal use), I'll make more. Period. |
#7
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Plant tissue cloning?
On Wed, 3 Mar 2004 11:09:36 +1100, "Peter Jason" wrote:
I would try the American patent office at first - there is a section on plants and cultivars - at http://patft.uspto.gov/netahtml/search-adv.htm These patents also have references to other patents and texts. Plus, I find the very IDEA of patening a plant or animal to be utterly idiotic-- and I don't care to hear about "business models". |
#8
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Plant tissue cloning?
No no!!!
I meant that the patents often refer to relevant texts which might be of use to you! For example, there is a recent US Patent.... United States Patent 6,696,619 Famodu , et al. February 24, 2004 "PLANT AMINOACYL-tRNA SYNTHASES" which has the following abstract.................... Abstract This invention relates to an isolated nucleic acid fragment encoding an aminoacyl-tRNA synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the aminoacyl-tRNA synthetase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the aminoacyl-tRNA synthetase in a transformed host cell. It is the "other references" below which might be of use... Other References Bork, P. Genome Research, vol. 10, p. 398-400, 2000.* Wolfgang Freist et al., Threonyl-tRNA Synthetase, Biol. Chem. Hoppe-Seyler, vol. 376, pp 213-224, Apr. 1995. Neidhart et al., (1975) Annu. Rev. Microbiol. 29:215-250. Eriani et al. (1990) Nature 347:203-206. Lloyd et al., (1995) Nucleic Acid Research 23(15):2882-2892. NCBI General Identifier No. 3319776. NCBI General Identifier No. 2501056. NCBI General Identifier No. 3617770. Plant Physiol. 121, 1053-1055 (1999) Nykiforuk et al. Ovesna et al., Plant Mitochondria, From Gene to Function: 139-142 (1998). Weygand-Durasevic, Nucleic Acid Research 15(5), 1887-1904 (1987) (Medline 87174725). Pape et al., Nucleic Acid Research 13(17), 6171-6183 (1985) (Medline 86016080). Note that patents are primarily LEGAL documents, so that the scientific bits will have to be checked thoroughly. "Darren Garrison" wrote in message ... On Wed, 3 Mar 2004 11:09:36 +1100, "Peter Jason" wrote: I would try the American patent office at first - there is a section on plants and cultivars - at http://patft.uspto.gov/netahtml/search-adv.htm These patents also have references to other patents and texts. Plus, I find the very IDEA of patening a plant or animal to be utterly idiotic-- and I don't care to hear about "business models". |
#9
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Plant tissue cloning?
Darren Garrison wrote in message . ..
Any good books or web sites with info on cloning plants from smallish tissue samples at home? I'm interested in trying to clone some rare-ish/expensive-ish venus flytrap and pitcher plant cultivars. Back in High School, I tried and failed cloning African Violets using one of the educational cloning kits that were just coming onto the market at the time. I've found references to several "cloning gel" products (mostly, apparently, on pothead sites). How useful would a product like this: http://www.hydroponics-garden.com/rootechgel4oz.html be for my purposes? My main worry is getting bacterial/fungal contamination in the medium-- and this claims to have "antimicrobials" in the medium. The cloning gel appears to be for regular size cuttings, not tissue culture or micropropagation. There are many books on plant tissue culture and also on carnivorous plants. Check google.com and amazon.com. Carnivorous Plant Tissue Culture http://www.flytrap.demon.co.uk/tc.htm There are a lot of carnivorous plant enthusiasts on the internet. Venus Flytrap Mutants http://hometown.aol.com/lulibybb/vftm.html International Carnivorous Plant Society http://www.carnivorousplants.org/ Tissue culture at home is challenging because unless you have thousands of dollars to spend, you have to make do without a laminar flow hood or build one yourself. You can substitute a pressure cooker for an autoclave to sterilize media and glassware. |
#10
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Plant tissue cloning?
Darren Garrison wrote in message . ..
Any good books or web sites with info on cloning plants from smallish tissue samples at home? I'm interested in trying to clone some rare-ish/expensive-ish venus flytrap and pitcher plant cultivars. Back in High School, I tried and failed cloning African Violets using one of the educational cloning kits that were just coming onto the market at the time. I've found references to several "cloning gel" products (mostly, apparently, on pothead sites). How useful would a product like this: http://www.hydroponics-garden.com/rootechgel4oz.html be for my purposes? My main worry is getting bacterial/fungal contamination in the medium-- and this claims to have "antimicrobials" in the medium. The cloning gel appears to be for regular size cuttings, not tissue culture or micropropagation. There are many books on plant tissue culture and also on carnivorous plants. Check google.com and amazon.com. Carnivorous Plant Tissue Culture http://www.flytrap.demon.co.uk/tc.htm There are a lot of carnivorous plant enthusiasts on the internet. Venus Flytrap Mutants http://hometown.aol.com/lulibybb/vftm.html International Carnivorous Plant Society http://www.carnivorousplants.org/ Tissue culture at home is challenging because unless you have thousands of dollars to spend, you have to make do without a laminar flow hood or build one yourself. You can substitute a pressure cooker for an autoclave to sterilize media and glassware. |
#11
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Plant tissue cloning?
Tissue culture at home is challenging because unless you have thousands of dollars to spend, you have to make do without a laminar flow hood or build one yourself. You can substitute a pressure cooker for an autoclave to sterilize media and glassware. Could equipment be sterilized in a normal microwave oven? |
#12
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Plant tissue cloning?
"David Hershey" wrote in message om... "Peter Jason" wrote in message ... Tissue culture at home is challenging because unless you have thousands of dollars to spend, you have to make do without a laminar flow hood or build one yourself. You can substitute a pressure cooker for an autoclave to sterilize media and glassware. Could equipment be sterilized in a normal microwave oven? You can't microwave metal instruments. You cannot get water above boiling in a microwave. Everything I have ever seen or read on plant tissue culture says you need a pressure cooker to sterilize water, media, etc. Here's more webpages on home tissue cultu http://www.une.edu.au/~agronomy/AgSSrHortTCinfo.html http://www.omnisterra.com/botany/cp/slides/tc/tc.htm http://www.kitchenculturekit.com/Index.htm Thanks for these pages - which are rather wonderful. My interest is grafting to produce chimeras, and possibly I can use tissue culture for this by microtoming slices of stock and scion, layering the slices, and developing them on media. Are there specific dyes for staining types of cells in a plant cross section? |
#13
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Plant tissue cloning?
"David Hershey" wrote in message om... "Peter Jason" wrote in message ... Tissue culture at home is challenging because unless you have thousands of dollars to spend, you have to make do without a laminar flow hood or build one yourself. You can substitute a pressure cooker for an autoclave to sterilize media and glassware. Could equipment be sterilized in a normal microwave oven? You can't microwave metal instruments. You cannot get water above boiling in a microwave. Everything I have ever seen or read on plant tissue culture says you need a pressure cooker to sterilize water, media, etc. Here's more webpages on home tissue cultu http://www.une.edu.au/~agronomy/AgSSrHortTCinfo.html http://www.omnisterra.com/botany/cp/slides/tc/tc.htm http://www.kitchenculturekit.com/Index.htm Thanks for these pages - which are rather wonderful. My interest is grafting to produce chimeras, and possibly I can use tissue culture for this by microtoming slices of stock and scion, layering the slices, and developing them on media. Are there specific dyes for staining types of cells in a plant cross section? |
#14
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Plant tissue cloning?
"Peter Jason" wrote in message ...
Tissue culture at home is challenging because unless you have thousands of dollars to spend, you have to make do without a laminar flow hood or build one yourself. You can substitute a pressure cooker for an autoclave to sterilize media and glassware. Could equipment be sterilized in a normal microwave oven? You can't microwave metal instruments. You cannot get water above boiling in a microwave. Everything I have ever seen or read on plant tissue culture says you need a pressure cooker to sterilize water, media, etc. Here's more webpages on home tissue cultu http://www.une.edu.au/~agronomy/AgSSrHortTCinfo.html http://www.omnisterra.com/botany/cp/slides/tc/tc.htm http://www.kitchenculturekit.com/Index.htm |
#15
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Plant tissue cloning?
Could equipment be sterilized in a normal microwave oven? BRBR
No Iris, Central NY, Zone 5a, Sunset Zone 40 "If we see light at the end of the tunnel, It's the light of the oncoming train." Robert Lowell (1917-1977) |
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