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Roller Drums for tissue culture; a question of rotation speed
OK I admit I'm an idiot dolt when it comes to stem props, but I do have the
memory of an elephant, and my friend Ed Wright never mentioned a shaker tables or any othe sort of table when he wrote his synopsis of stem props for OrchidSafari http://www.geocities.com/~marylois/archiv23.html Granted you may be doing these en masse, but even still.... K Barrett "Pat Brennan" wrote in message ... Hey Al, Seems we are going down about the same route. I have been playing around with this for about 9 months with limited success. I found Arditti a good starting point but have really just been winging it once I got started. One thing going in our favor is that there are a couple of companies selling premixed medias. The web site for PhytoTech NZ or Aust was helpful in suggesting changes to the standard mult mix when using it for Phals. Both G&B and PhytoTech said they had someone who could help me, but in both cases I have been unable to reach these people (always out of country or not in today). I have a shaker table so I really am not much help with the drum speed. I assumed the low speed unit was what you wanted until I looked at the speed I run the shaker at. ( I know the speed by the sound and how the tissue moves in the flask and not by rpm.) Any ways I tend to run the shaker at 60 to 100 rpm. This is about the same speed I use for cloning Cats and Oncs. Given that speed, I am not sure the tissue will roll in the culture tubes with the low speed drum, I would call the company before buying either. That being said, I have not used the shaker for the last 4 or 5 months because I was having better luck with solid media. One of the protocols in Arditti starts in a shaker, but quickly moves to solid media --I guess this is the route I have gone. Funny but I am having better luck with older media. I get my best multiplication after a dark ring has formed around the tissue. I have yet to kill the tissue from the phenyols but I am sure I will push it too long one of these days. Currently I am learning by trial and error the size the tissue should be before dividing and where the dividing should be done. I was losing a fair amount of tissue after cutting it up. I cut back on dividing and really slowed the process down. I have yet to find a balance I am happy with and wonder if the freshly cut tissue should be treated with something before being placed back on the media. Do not be too hard on yourself when measuring your success. I have found that when sending Phals out for cloning, a batch of 500 plants will take 2 to 3 years before flasks are returned. Pat "Al" wrote in message news My goal is to expand my experience with stem propagation of Phals by actually excising the node and attempting to multiply the meristematic tissue in a liquid media but I probably won't stop there. I was thinking of a roller drum because it looks neat. :-) A shaker table is probably more within my budget. Are Phals orchids which like or dislike a 24 hour light cycle during the multiplication phase? I have a book called Micropropagation of Orchids by Arditti and Ernst published in 1993. It outlines research on propagation techniques for many orchids by genera and provides a kind of history of propagation for most but I have been told several times that it is dated and newer info is available to augment what was compiled in this book. I don't have this newer info at my fingertips or really know where to get it. :-( Anyway, dated or not, I will turn to it once I assemble all the little gadgets I need for this mad experiment. Thank you to everybody who offered input. If there is more to come I will be on the look out for it. "Aaron Hicks" wrote in message ... Al, you don't specify what you want to use it for, but the upper speed is going to be way too high for tissue culture work with orchids. Keep in mind that some orchids don't like 24 hr/day cycles for tumbling. Some do best with a few hours on, followed by a few hours off. I think there are plans on the web somewhere for do-it-yourself rigs; ditto with eBay and Labx. Save some bucks. -AJHicks Chandler, AZ |
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