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Roller Drums for tissue culture; a question of rotation speed
Al wrote:
I wanted a shaker table so I could try a better method of making the callus tissue proliferation "peanut" more reliably, and from my reading I think this will involve agitation. And for agitation you need a liquid media and some form of roller/shaker movement. There is a guy in my local society who has nothing better to do than go to his test tubes every few hours for weeks or months and shake them up by hand and for him this works to sufficiently agitate the sample. I'd get tired of that really quickly and when confronted with this option the price of the roller drum/shaker tables from New Brunswick Scientific Equipment's catalog looks like the more reasonable option although they all take my breath away. I am glad these are not the only choices... If you are just doing a few tubes or flasks, there are models which aren't much bigger than a piece of writing paper (the tops of them, anyway). That might be a more reasonable option than the big chest shakers. We had one (admittedly at least 30 years old) the size of a big chest freezer. Of course it broke down a couple times a month... You can make one. You just need an electric motor and a camshaft. Come to think of it, a random orbit sander could be adapted to make a shaker table pretty easily. Just turn it upside down, screw a platform on it, and use the trigger lock to keep it turned on. I wonder how long the motor would last... That might be my best idea this week, and I have had a couple good ones, not that I ever remember to write them down (good idea number 4 - write down good ideas). Rob -- Rob's Rules: http://www.msu.edu/~halgren 1) There is always room for one more orchid 2) There is always room for two more orchids 2a. See rule 1 3) When one has insufficient credit to purchase more orchids, obtain more credit |
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